Journal: Biomicrofluidics

Article Title: On-chip immuno-agglutination assay based on a dynamic magnetic bead clump and a sheath-less flow cytometry

doi: 10.1063/1.5093766

Figure Lengend Snippet: Dose-response curve of the antigen/antibody assay for IgG. (a) Percentage of monomers vs sample concentration of both rabbit IgG and negative control and (b) normalized signal vs concentration of rabbit IgG. Each dot is the average result of five measurements, and the error bars represent SD.

Article Snippet: In the second set of experiments, goat anti-rabbit IgG coated MBs are prepared by adding protein A-coated MBs suspension of 5 μ l (Dynabeads Protein A for Immunoprecipitation, ThermoFisher Scientific Co., USA) to 10 μ g goat anti-rabbit IgG (bs-0295G, Bioss Antibodies Co., China) diluted in 40 μ l PBS with 0.1% Tween-20, followed by surface-sealing with goat serum (C-0005, Bioss Antibodies Co., China) of 50 μ l. The processed rabbit IgG (bs-0295P, Bioss Antibodies Co., China) is then diluted by factors of 103–108 with PBS and used as analyte.

Techniques: Concentration Assay, Negative Control

Journal: The Journal of Neuroscience

Article Title: Nerve terminal nAChRs initiate quantal GABA release from perisomatic interneurons by activating axonal T-type (Ca v 3) Ca 2+ channels and Ca 2+ release from stores

doi: 10.1523/JNEUROSCI.2781-11.2011

Figure Lengend Snippet: Primary antibodies, dilutions and sources used in experiments (see Methods and Materials), a sample reference in which the specificity the antibody has been demonstrated, and the method used in that reference to validate the specificity of the antibody.

Article Snippet: Ca v 3.1 , Rabbit IgG , 1:100 , Alomone ACC-021 , Ernst W.L., Zhang Y., Yoo J.W., Ernst S.J. and Noebels J.L. 2009. Genetic enhancement of thalamocortical network activity by elevating α-1G mediated low-voltage-activated calcium current induces pure absence epilepsy. J Neurosci 29:1615–25. , Western blot of brain lysates from transgenic mice over-expressing receptor; validated against Ca v 3.1 KO mouse (T. Snutch, pers. comm..

Techniques: Binding Assay, Western Blot, Activity Assay, Transgenic Assay